RRC ID 1710
著者 Carré C, Szymczak D, Pidoux J, Antoniewski C.
タイトル The histone H3 acetylase dGcn5 is a key player in Drosophila melanogaster metamorphosis.
ジャーナル Mol Cell Biol
Abstract Although it has been well established that histone acetyltransferases (HATs) are involved in the modulation of chromatin structure and gene transcription, there is only little information on their developmental role in higher organisms. Gcn5 was the first transcription factor with HAT activity identified in eukaryotes. Here we report the isolation and characterization of Drosophila melanogaster dGcn5 mutants. Null dGcn5 alleles block the onset of both oogenesis and metamorphosis, while hypomorphic dGcn5 alleles impair the formation of adult appendages and cuticle. Strikingly, the dramatic loss of acetylation of the K9 and K14 lysine residues of histone H3 in dGcn5 mutants has no noticeable effect on larval tissues. In contrast, strong cell proliferation defects in imaginal tissues are observed. In vivo complementation experiments revealed that dGcn5 integrates specific functions in addition to chromosome binding and acetylation. Surprisingly, a dGcn5 variant protein with a deletion of the bromodomain, which has been shown to recognize acetylated histones, appears to be fully functional. Our results establish dGcn5 as a major histone H3 acetylase in Drosophila which plays a key role in the control of specific morphogenetic cascades during developmental transitions.
巻・号 25(18)
ページ 8228-38
公開日 2005-9-1
DOI 10.1128/MCB.25.18.8228-8238.2005
PII 25/18/8228
PMID 16135811
PMC PMC1234334
MeSH Acetylation Acetyltransferases / genetics Acetyltransferases / metabolism* Alleles Animals Cell Proliferation Chromosomes / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology* Drosophila melanogaster / growth & development* Histone Acetyltransferases Histones / metabolism Larva / enzymology Larva / growth & development Lysine / metabolism Metamorphosis, Biological* / genetics Mutation Oogenesis / genetics Protein Structure, Tertiary / genetics RNA Interference Sequence Deletion
IF 3.611
引用数 61
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
情報 KYOTO-Fly
ショウジョウバエ NP5153 (DGRC#113604)