RRC ID 2441
著者 Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, Wataya T, Takahashi JB, Nishikawa S, Nishikawa S, Muguruma K, Sasai Y.
タイトル A ROCK inhibitor permits survival of dissociated human embryonic stem cells.
ジャーナル Nat Biotechnol
Abstract Poor survival of human embryonic stem (hES) cells after cell dissociation is an obstacle to research, hindering manipulations such as subcloning. Here we show that application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency (from approximately 1% to approximately 27%) and facilitates subcloning after gene transfer. Furthermore, dissociated hES cells treated with Y-27632 are protected from apoptosis even in serum-free suspension (SFEB) culture and form floating aggregates. We demonstrate that the protective ability of Y-27632 enables SFEB-cultured hES cells to survive and differentiate into Bf1(+) cortical and basal telencephalic progenitors, as do SFEB-cultured mouse ES cells.
巻・号 25(6)
ページ 681-6
公開日 2007-6-1
DOI 10.1038/nbt1310
PII nbt1310
PMID 17529971
MeSH Amides / administration & dosage* Animals Cell Differentiation / drug effects* Cell Survival / drug effects* Cells, Cultured Dose-Response Relationship, Drug Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism* Humans Intracellular Signaling Peptides and Proteins / antagonists & inhibitors* Intracellular Signaling Peptides and Proteins / metabolism* Mice Protein Serine-Threonine Kinases / antagonists & inhibitors* Protein Serine-Threonine Kinases / metabolism* Pyridines / administration & dosage* rho-Associated Kinases
IF 36.553
引用数 1225
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒトES細胞 KhES-1 KhES-2 KhES-3