RRC ID 43896
著者 Adfa M, Itoh T, Hattori Y, Koketsu M.
タイトル Inhibitory effects of 6-alkoxycoumarin and 7-alkoxycoumarin derivatives on lipopolysaccharide/interferon γ-stimulated nitric oxide production in RAW264 cells.
ジャーナル Biol Pharm Bull
Abstract Coumarin and its derivatives are well known for their anti-inflammatory and anti-oxidative effects. In this study, we synthesized 32 coumarin derivatives from commercially available 6-hydroxycoumarin (6HC) and 7-hydroxycoumarin (7HC) and examined their effects on lipopolysaccharide/interferon γ (LPS/IFNγ)-stimulated nitric oxide (NO) production in murine macrophage RAW264 cells. Among these derivatives, 6HC-8 (6-(3-phenylpropoxy)coumarin), 6HC-14 (6-(2-octynyloxy)coumarin), 7HC-14 (7-(2-octynyloxy)coumarin), and 7HC-16 (7-(3,5-dimethoxybenzyloxy)coumarin) markedly suppressed NO production at low concentration (25 µM). These synthesized coumarin derivatives also markedly inhibited inducible NO synthase (iNOS) protein and mRNA expression, as assessed by western blotting and quantitative real time-polymerase chain reaction (RT-PCR).
巻・号 35(6)
ページ 963-6
公開日 2012-1-1
DOI 10.1248/bpb.35.963
PII DN/JST.JSTAGE/bpb/35.963
PMID 22687539
MeSH Animals Cell Line Coumarins / pharmacology* Interferon-gamma / pharmacology Lipopolysaccharides / pharmacology Mice Nitric Oxide / antagonists & inhibitors* Nitric Oxide / metabolism Nitric Oxide Synthase Type II / antagonists & inhibitors* Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / metabolism RNA, Messenger / metabolism
IF 1.863
引用数 6
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)