RRC ID 41178
著者 Guo L, Zhao J, Liu C, Bai L, Sun P, Han C, Li J, Guo X, Xiang W, Wang X.
タイトル Sphaerisporangium aureirubrum sp. nov., an actinomycete isolated from soil.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel actinomycete, designated strain NEAU-GQTH1-3T, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and forms a stable clade with its closest relative Sphaerisporangium rubeum JCM 13067T (98.2 % 16S rRNA gene sequence similarity). Moreover, morphological and chemotaxonomic properties of strain NEAU-GQTH1-3T also confirmed the affiliation of the isolate to the genus Sphaerisporangium. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, galactose, madurose, mannose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The major menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The predominant cellular fatty acids were iso-C16 : 0 and 10-methyl C17 : 0. Mycolic acids were absent. The DNA G+C content was 70.4 mol%. However, the low level of DNA-DNA relatedness and some phenotypic characteristics allowed the isolate to be differentiated from its closest relative. Therefore, it is concluded that strain NEAU-GQTH1-3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium aureirubrum sp. nov. is proposed. The type strain is NEAU-GQTH1-3T ( = CGMCC 4.7199T = JCM 30346T).
巻・号 65(11)
ページ 4157-4162
公開日 2015-11-1
DOI 10.1099/ijsem.0.000554
PMID 26303767
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification Bacterial Typing Techniques Base Composition Cell Wall / chemistry China DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 2
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 13067 JCM 30346