RRC ID |
35542
|
著者 |
Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Malihan LB, Lee WK, Park SJ, Chung WJ.
|
タイトル |
Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate.
|
ジャーナル |
Bioprocess Biosyst Eng
|
Abstract |
D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L(-1) D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L(-1) D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L(-1) and yield of 62.0 %. Batch fermentation in 20 g L(-1) D-galactose of E. coli ∆galK∆dgoK mutant expressing the gld resulted in 17.6 g L(-1) of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.
|
巻・号 |
37(3)
|
ページ |
383-91
|
公開日 |
2014-3-1
|
DOI |
10.1007/s00449-013-1003-6
|
PMID |
23820824
|
MeSH |
Base Sequence
Cloning, Molecular
Culture Media
DNA Primers
Escherichia coli / genetics
Escherichia coli / metabolism*
Galactose Dehydrogenases / genetics
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecular Structure
Pseudomonas syringae / enzymology
Sugar Acids / chemistry
Sugar Acids / metabolism*
|
IF |
2.419
|
引用数 |
5
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
ENGINEERING, CHEMICAL
|
リソース情報 |
原核生物(大腸菌) |
ME9062(BW25113)
JW0740-KC(galK) |