RRC ID 35634
著者 Aoki W, Saito M, Manabe R, Mori H, Yamaguchi Y, Tamiya E.
タイトル Integrating reductive and synthetic approaches in biology using man-made cell-like compartments.
ジャーナル Sci Rep
Abstract We propose 'integrated synthetic genetics' as a novel methodology that integrates reductive and synthetic approaches used in life science research. Integrated synthetic genetics enables determinations of sets of genes required for the functioning of any biological subsystem. This method utilizes artificial cell-like compartments, including a randomly introduced whole gene library, strictly defined components for in vitro transcription and translation and a reporter that fluoresces 'only when a particular function of a target biological subsystem is active.' The set of genes necessary for the target biological subsystem can be identified by isolating fluorescent artificial cells and multiplex next-generation sequencing of genes included in these cells. The importance of this methodology is that screening for the set of genes involved in a subsystem and reconstructing the entire subsystem can be done simultaneously. This methodology can be applied to any biological subsystem of any species and may remarkably accelerate life science research.
巻・号 4
ページ 4722
公開日 2014-4-17
DOI 10.1038/srep04722
PII srep04722
PMID 24740007
PMC PMC3989557
MeSH Artificial Cells / chemistry* Artificial Cells / cytology Cell Compartmentation* Protein Biosynthesis Synthetic Biology* Transcription, Genetic
IF 3.998
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ASKA library