RRC ID |
41041
|
著者 |
Liu C, Guan X, Jin P, Li J, Li Y, Li L, Zhou Y, Shen Y, Wang X, Xiang W.
|
タイトル |
Dactylosporangium cerinum sp. nov., a novel actinobacterium isolated from the rhizosphere of Pinus koraiensis Sieb. et Zucc.
|
ジャーナル |
Antonie Van Leeuwenhoek
|
Abstract |
A novel actinobacterium, designated strain NEAU-TPG4(T) was isolated from rhizosphere soil of Pinus koraiensis Sieb. et Zucc. collected from Luobei, Heilongjiang Province, north China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-TPG4(T) were consistent with the description of the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan consisted of meso- and 3-hydroxy-diaminopimelic acids; arabinose, xylose and glucose were found as whole-cell sugars. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were identified as iso-C16:0, anteiso-C15:0, anteiso-C17:0 and C18:0. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relatives were Dactylosporangium maewongense Japan Collection of Microorganism (JCM) 15933(T) (99.6 %), Dactylosporangium siamense NBRC 106093(T) (99.6 %), Dactylosporangium aurantiacum JCM 3083(T) (99.5 %) and Dactylosporangium luteum JCM 17685(T) (99.5 %). However, the low level of DNA-DNA relatedness and several cultural and physiological characteristics allowed the strain to be distinguished from its closest relatives. Thus, it is proposed that strain NEAU-TPG4(T) represents a novel Dactylosporangium species. Dactylosporangium cerinum sp. nov. The type strain of D. cerinum is NEAU-TPG4(T) (=CGMCC 4.7152(T) = DSM 46712(T)).
|
巻・号 |
108(1)
|
ページ |
191-9
|
公開日 |
2015-7-1
|
DOI |
10.1007/s10482-015-0478-3
|
PMID |
25987396
|
MeSH |
Carbohydrates / analysis
Cell Wall / chemistry
China
Cluster Analysis
Cytosol / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Diaminopimelic Acid / analysis
Fatty Acids / analysis
Japan
Micromonosporaceae / classification*
Micromonosporaceae / genetics
Micromonosporaceae / isolation & purification*
Micromonosporaceae / physiology
Molecular Sequence Data
Nucleic Acid Hybridization
Peptidoglycan / analysis
Phospholipids / analysis
Phylogeny
Pinus / microbiology*
RNA, Ribosomal, 16S / genetics
Rhizosphere*
Sequence Analysis, DNA
Soil Microbiology*
Spores, Bacterial / cytology
Vitamin K 2 / analysis
|
IF |
2.674
|
引用数 |
0
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 15933
JCM 3083
JCM 17685 |