RRC ID 41089
著者 Singh P, Kim YJ, Hoang VA, Farh Mel-A, Yang DC.
タイトル Sphingomonas panacis sp. nov., isolated from rhizosphere of rusty ginseng.
ジャーナル Antonie Van Leeuwenhoek
Abstract The type strain DCY99(T) was isolated from soil collected from a ginseng field in Hwacheon, Republic of Korea. Strain DCY99(T) is Gram-negative, non-spore forming, motile, rod-shaped, and strictly aerobic. The bacteria grow optimally at 25-30 °C and pH 6.0-6.5. Phylogenetically, strain DCY99(T) is most closely related to Sphingomonas oligophenolica JCM 12082(T), followed by Sphingomonas asaccharolytica KCTC 2825(T), Sphingomonas mali KCTC 2826(T), Sphingomonas cynarae JCM17498(T), Sphingomonas pruni KCTC 2824(T), and Sphingomonas glacialis DSM 22294(T). The DNA-DNA relatedness between strain DCY99(T) and S. oligophenolica JCM 12082(T) was 15.6 ± 0.4 %, and the DNA G+C content of strain DCY99(T) was 64.4 mol%. An isoprenoid quinone was detected and identified as ubiquinone Q-10, and sym-homospermidine was identified as the major polyamine of DCY99(T). The major polar lipids were identified as sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylcholine. C14:02OH, C16:0, and summed feature 8 (C18:1 ω7c:/C18:1 ω6c) were identified as the major fatty acids present in DCY99(T). The results of physiological and biochemical tests allowed strain DCY99(T) to be differentiated phenotypically from other recognized species belonging to the genus Sphingomonas. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Sphingomonas panacis sp. nov. is proposed with the type strain designated as DCY99(T) (=JCM 30806(T) =KCTC 42347(T)).
巻・号 108(3)
ページ 711-20
公開日 2015-9-1
DOI 10.1007/s10482-015-0527-y
PMID 26155772
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Hydrogen-Ion Concentration Locomotion Nucleic Acid Hybridization Panax / microbiology* Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Republic of Korea Rhizosphere Sequence Analysis, DNA Soil Microbiology* Spermidine / analysis Sphingomonas / classification* Sphingomonas / genetics Sphingomonas / isolation & purification* Sphingomonas / physiology Temperature
IF 2.674
引用数 13
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17498 JCM 12082 JCM 30806