RRC ID 36710
著者 Suzaki T, Ito M, Yoro E, Sato S, Hirakawa H, Takeda N, Kawaguchi M.
タイトル Endoreduplication-mediated initiation of symbiotic organ development in Lotus japonicus.
ジャーナル Development
Abstract Many leguminous plants have a unique ability to reset and alter the fate of differentiated root cortical cells to form new organs of nitrogen-fixing root nodules during legume-Rhizobium symbiosis. Recent genetic studies on the role of cytokinin signaling reveal that activation of cytokinin signaling is crucial to the nodule organogenesis process. However, the genetic mechanism underlying the initiation of nodule organogenesis is poorly understood due to the low number of genes that have been identified. Here, we have identified a novel nodulation-deficient mutant named vagrant infection thread 1 (vag1) after suppressor mutant screening of spontaneous nodule formation 2, a cytokinin receptor gain-of-function mutant in Lotus japonicus. The VAG1 gene encodes a protein that is putatively orthologous to Arabidopsis ROOT HAIRLESS 1/HYPOCOTYL 7, a component of the plant DNA topoisomerase VI that is involved in the control of endoreduplication. Nodule phenotype of the vag1 mutant shows that VAG1 is required for the ploidy-dependent cell growth of rhizobial-infected cells. Furthermore, VAG1 mediates the onset of endoreduplication in cortical cells during early nodule development, which may be essential for the initiation of cortical cell proliferation that leads to nodule primordium formation. In addition, cortical infection is severely impaired in the vag1 mutants, whereas the epidermal infection threads formation is normal. This suggests that the VAG1-mediated endoreduplication of cortical cells may be required for the guidance of symbiotic bacteria to host meristematic cells.
巻・号 141(12)
ページ 2441-5
公開日 2014-6-1
DOI 10.1242/dev.107946
PII dev.107946
PMID 24850853
MeSH Archaeal Proteins / genetics Archaeal Proteins / physiology* Bacteria Cell Lineage Cytokinins / metabolism DNA Topoisomerases, Type II / genetics DNA Topoisomerases, Type II / physiology* Endoreduplication / physiology* Gene Expression Regulation, Plant* Genes, Plant Histidine Kinase Lotus / embryology* Lotus / microbiology Lotus / physiology* Meristem / embryology Meristem / microbiology Mutation Phenotype Plant Epidermis / metabolism Plant Proteins / genetics Plant Proteins / physiology Plants, Genetically Modified Promoter Regions, Genetic Protein Kinases / genetics Protein Kinases / physiology* Root Nodules, Plant / microbiology Root Nodules, Plant / physiology Signal Transduction Symbiosis
IF 5.611
引用数 26
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ミヤコグサ・ダイズ M2 Bulked seed