RRC ID 47700
著者 Goparaju SK, Kohda K, Ibata K, Soma A, Nakatake Y, Akiyama T, Wakabayashi S, Matsushita M, Sakota M, Kimura H, Yuzaki M, Ko SB, Ko MS.
タイトル Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors.
ジャーナル Sci Rep
Abstract Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.
巻・号 7
ページ 42367
公開日 2017-2-13
DOI 10.1038/srep42367
PII srep42367
PMID 28205555
PMC PMC5304326
MeSH Biomarkers / metabolism Cell Differentiation / genetics* Cell Shape Humans Ion Channels / metabolism Kinetics Motor Neurons / cytology* Motor Neurons / metabolism Neurogenesis / genetics Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Transcription Factors / genetics* Transcription Factors / metabolism
IF 3.998
引用数 26
WOS 分野 NEUROSCIENCES
リソース情報
ヒト・動物細胞 201B7(HPS0063)