| RRC ID |
36681
|
| 著者 |
Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
|
| タイトル |
Cellular Dynamics of Mouse Trophoblast Stem Cells: Identification of a Persistent Stem Cell Type.
|
| ジャーナル |
Biol Reprod
|
| Abstract |
Mouse trophoblast stem cells (TSCs) proliferate indefinitely in vitro, despite their highly heterogeneous nature. In this study, we sought to characterize TSC colony types by using methods based on cell biology and biochemistry for a better understanding of how TSCs are maintained over multiple passages. Colonies of TSCs could be classified into four major types: type 1 is compact and dome-shaped, type 4 is flattened but with a large multilayered cell cluster, and types 2 and 3 are their intermediates. A time-lapse analysis indicated that type 1 colonies predominantly appeared after passaging, and a single type 1 colony gave rise to all other types. These colony transitions were irreversible, but at least some type 1 colonies persisted throughout culture. The typical cells comprising type 1 colonies were small and highly motile, and they aggregated together to form primary colonies. A hierarchical clustering based on global gene expression profiles suggested that a TSC line containing more type 1 colony cells was similar to in vivo extraembryonic tissues. Among the known TSC genes examined, Elf5 showed a differential expression pattern according to colony type, indicating that this gene might be a reliable marker of undifferentiated TSCs. When aggregated with fertilized embryos, cells from types 1 and 2, but not from type 4, distributed to the polar trophectoderm in blastocysts. These findings indicate that cells typically found in type 1 colonies can persist indefinitely as stem cells and are responsible for the maintenance of TSC lines. They may provide key information for future improvements in the quality of TSC lines.
|
| 巻・号 |
94(6)
|
| ページ |
122
|
| 公開日 |
2016-6-1
|
| DOI |
10.1095/biolreprod.115.137125
|
| PII |
biolreprod.115.137125
|
| PMID |
27122635
|
| PMC |
PMC6702784
|
| MeSH |
Animals
Cell Differentiation
Cell Line
DNA-Binding Proteins / metabolism
Female
Male
Mice, Inbred C57BL
Mice, Inbred DBA
Stem Cells / cytology*
Stem Cells / metabolism
Transcription Factors / metabolism
Trophoblasts / cytology*
Trophoblasts / metabolism
|
| IF |
3.322
|
| 引用数 |
5
|
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00267 |
