RRC ID 41208
著者 Liu Y, Lai Q, Göker M, Meier-Kolthoff JP, Wang M, Sun Y, Wang L, Shao Z.
タイトル Genomic insights into the taxonomic status of the Bacillus cereus group.
ジャーナル Sci Rep
Abstract The identification and phylogenetic relationships of bacteria within the Bacillus cereus group are controversial. This study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based Genome BLAST Distance Phylogeny (GBDP) approach. The GBDP analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. Additionally, 16S rRNA gene analysis, a novel variant of multi-locus sequence analysis (nMLSA) and screening of virulence genes were performed. The 16S rRNA gene sequence was not sufficient to differentiate the bacteria within this group due to its high conservation. The nMLSA results were consistent with GBDP. Moreover, a fast typing method was proposed using the pycA gene, and where necessary, the ccpA gene. The pXO plasmids and cry genes were widely distributed, suggesting little correlation with the phylogenetic positions of the host bacteria. This might explain why classifications based on virulence characteristics proved unsatisfactory in the past. In summary, this is the first large-scale and systematic study of the taxonomic status of the bacteria within the B. cereus group using whole-genome sequences, and is likely to contribute to further insights into their pathogenicity, phylogeny and adaptation to diverse environments.
巻・号 5
ページ 14082
公開日 2015-9-16
DOI 10.1038/srep14082
PII srep14082
PMID 26373441
PMC PMC4571650
MeSH Bacillus / classification* Bacillus / genetics* Bacillus / pathogenicity DNA Barcoding, Taxonomic* Genes, Bacterial Genome, Bacterial* Genomics* / methods High-Throughput Nucleotide Sequencing Multilocus Sequence Typing Phylogeny RNA, Ribosomal, 16S / genetics Virulence Factors / genetics
IF 3.998
引用数 106
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 15802 JCM 15801