RRC ID 41046
著者 Shimoshige H, Kobayashi H, Mizuki T, Nagaoka Y, Inoue A, Maekawa T.
タイトル Effect of Polyethylene Glycol on the Formation of Magnetic Nanoparticles Synthesized by Magnetospirillum magnetotacticum MS-1.
ジャーナル PLoS One
Abstract Magnetotactic bacteria (MTB) synthesize intracellular magnetic nanocrystals called magnetosomes, which are composed of either magnetite (Fe3O4) or greigite (Fe3S4) and covered with lipid membranes. The production of magnetosomes is achieved by the biomineralization process with strict control over the formation of magnetosome membrane vesicles, uptake and transport of iron ions, and synthesis of mature crystals. These magnetosomes have high potential for both biotechnological and nanotechnological applications, but it is still extremely difficult to grow MTB and produce a large amount of magnetosomes under the conventional cultural conditions. Here, we investigate as a first attempt the effect of polyethylene glycol (PEG) added to the culture medium on the increase in the yield of magnetosomes formed in Magnetospirillum magnetotacticum MS-1. We find that the yield of the formation of magnetosomes can be increased up to approximately 130 % by adding PEG200 to the culture medium. We also measure the magnetization of the magnetosomes and find that the magnetosomes possess soft ferromagnetic characteristics and the saturation mass magnetization is increased by 7 %.
巻・号 10(5)
ページ e0127481
公開日 2015-1-1
DOI 10.1371/journal.pone.0127481
PII PONE-D-14-55070
PMID 25993286
PMC PMC4439050
MeSH Culture Media / pharmacology Magnetite Nanoparticles / chemistry* Magnetite Nanoparticles / ultrastructure Magnetosomes / drug effects Magnetosomes / ultrastructure Magnetospirillum / drug effects Magnetospirillum / growth & development Magnetospirillum / metabolism* Polyethylene Glycols / pharmacology*
IF 2.74
引用数 4
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 21281