RRC ID 11050
著者 Tatematsu K, Yamamoto K, Uchino K, Narukawa J, Iizuka T, Banno Y, Katsuma S, Shimada T, Tamura T, Sezutsu H, Daimon T.
タイトル Positional cloning of silkworm white egg 2 (w-2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects.
ジャーナル Genes Cells
Abstract The white, scarlet and brown genes of Drosophila melanogaster encode three half-type ATP-binding cassette (ABC) transporters. In Drosophila, precursors of ommochromes and pteridines are transported by White/Scarlet and White/Brown heterodimers, respectively. The white egg 2 (w-2) mutant of the silkworm, Bombyx mori, has white eggs and eyes because of lack of ommochrome granules in the serosa and eyes. Here, we report that the silkworm w-2 locus encodes an ortholog of Drosophila scarlet. Our results indicate that Bombyx Scarlet forms a heterodimer with Bombyx White to transport ommochrome precursors, suggesting that formation of a White/Scarlet heterodimer and its involvement in the transport of ommochrome precursors are evolutionarily ancient and widely conserved traits in insects. Contrary to dipteran insects, white and scarlet were juxtaposed in a head-to-tail orientation in the silkworm genome, suggesting that the origin of white and scarlet was a tandem duplication of their ancestral transporter gene. In Bombyx, White is also essential for the transport of uric acid in larval epidermis. However, our results suggest that a Bombyx White/Scarlet heterodimer is not involved in this process. Our results emphasize the functional conservation and diversification of half-type ABC transporter families in insects, which may contribute to their extremely diverse color patterns.
巻・号 16(4)
ページ 331-42
公開日 2011-4-1
DOI 10.1111/j.1365-2443.2011.01490.x
解説 Bm-w-2は、ABCトランスポーターfamilyに属するタンパク質をコードする。w-2を保有するe06系統とe15系統では、Bm-w-2のORF内に7bpの欠失が存在する。w-2rを保有するe16系統では、Bm-w-2のORF内に6bpの欠失が存在する。
PMID 21294818
MeSH ATP-Binding Cassette Transporters / genetics* Animals Bombyx / genetics* Bombyx / metabolism Cloning, Molecular DNA Barcoding, Taxonomic Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Gene Expression Profiling* Genetic Association Studies Insecta / genetics Molecular Sequence Data Mutation Phylogeny Pigmentation / genetics*
IF 1.655
引用数 39
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
カイコ p50 e06 e15 e16 t90