| RRC ID |
11245
|
| 著者 |
Fujii T, Abe H, Katsuma S, Shimada T.
|
| タイトル |
Identification and characterization of the fusion transcript, composed of the apterous homolog and a putative protein phosphatase gene, generated by 1.5-Mb interstitial deletion in the vestigial (Vg) mutant of Bombyx mori.
|
| ジャーナル |
Insect Biochem Mol Biol
|
| Abstract |
The vestigial (Vg) mutant is a Z-linked mutant that causes vestigial wings in the silkworm, Bombyx mori. We have previously reported a 1.5-Mb interstitial deletion on the Z chromosome bearing the Vg mutation (Z(Vg) chromosome). In this study, we found that exons 3-8 of a gene named Bmptp-Z encoding a putative tyrosine-specific protein phosphatase are deleted by the 1.5-Mb interstitial deletion. We found that a gene encoding the Bombyx homolog of Drosophila Apterous (BmAp-A) protein is located 4.5 kb downstream of the distal breakpoint of the 1.5-Mb interstitial deletion. Moreover, an in-frame fusion transcript composed of the 5' part of Bmptp-Z and the 3' part of Bmap-A is generated specific to the Z(Vg) chromosome. Effects of the in-frame fusion transcript on the vestigial phenotype are discussed.
|
| 巻・号 |
41(5)
|
| ページ |
306-12
|
| 公開日 |
2011-5-1
|
| DOI |
10.1016/j.ibmb.2011.01.007
|
| PII |
S0965-1748(11)00024-5
|
| 解説 |
Vg(痕跡翅）の原因遺伝子は、Bmap-Aである。Bmap-Aは、ショウジョウバエのapterousのカイコホモログである。Vg系統(u01)のZ染色体には、1.8 Mbの中間部欠失が存在する。この欠失が原因で、通常は1.8 Mb離れている二つの遺伝子(Bmptp-ZとBmap-A)から融合転写産物が生じる。
|
| PMID |
21296154
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Bombyx / anatomy & histology
Bombyx / genetics*
Chromosome Breakpoints
Chromosome Mapping
Drosophila
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism*
Exons
Gene Expression
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism*
Insect Proteins / genetics*
Insect Proteins / metabolism
LIM-Homeodomain Proteins
Molecular Sequence Data
Mutation
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Phenotype
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism*
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Recombinant Fusion Proteins / genetics*
Recombinant Fusion Proteins / metabolism
Sequence Alignment
Sequence Deletion
Sequence Homology, Amino Acid
Sex Chromosomes / physiology
Transcription Factors / genetics
Transcription Factors / metabolism*
Wings, Animal / anatomy & histology
|
| IF |
3.827
|
| 引用数 |
1
|
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
u01 |
