RRC ID 11260
著者 Yamamoto K, Aso Y, Fujii H.
タイトル Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori.
ジャーナル J Insect Sci
Abstract The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and specifically to MCM7, of vertebrates and invertebrates. An RT-PCR showed that the bmMCM7 transcript was present in the ovaries, testes, silk glands, and fat bodies of larval silkworms. Expression plasmids were transformed into competent Escherichia coli and overexpressed. This is the first report on the identification of MCM helicase of the silkworm, B. mori.
巻・号 10
ページ 148
公開日 2010-1-1
DOI 10.1673/031.010.14108
PMID 21070176
PMC PMC3016899
MeSH Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Cloning, Molecular Cluster Analysis DNA Helicases / genetics* DNA Primers / genetics DNA, Complementary / genetics Escherichia coli Genetic Vectors / genetics Molecular Sequence Data Nuclear Proteins / genetics* Phylogeny* Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Species Specificity
IF 1.325
引用数 0
WOS 分野 ENTOMOLOGY
リソース情報
カイコ p50