論文 - 詳細
| RRC ID | 12162 |
|---|---|
| 著者 | Takakuwa T, Endo S, Shirakura Y, Yokoyama M, Tamatani M, Tohyama M, Aozasa K, Inada K. |
| タイトル | Interleukin-10 gene transfer improves the survival rate of mice inoculated with Escherichia coli. |
| ジャーナル | Crit Care Med |
| Abstract |
OBJECTIVE:To determine whether administration of recombinant adenovirus vectors encoding the interleukin (IL)-10 protein (AxCAmIL-10) decreases the mortality of septic mice. DESIGN:Prospective, randomized, controlled study. SETTING:University research laboratory. SUBJECTS:Adult male C57B/6 mice. INTERVENTIONS:Untreated mice and those injected intraperitoneally with 1 x 10(9) pfu of AxCAmIL-10 were used as control 1 and 2, respectively. Double-capsules without Escherichia coli were intraperitoneally embedded in another group (control 3). Mice embedded with capsules containing E. coli were divided into the following groups: simultaneous administration of 0.5 mL of saline (group 1), and administration of AxCAmIL-10 3 hrs before embedding (group 2) or 1 hr after embedding (group 3). Histopathologic changes together with expression concentrations of IL-10 and tumor necrosis factor (TNF) in various organs and plasma were examined 18 hrs after each treatment. Observation periods were 5-8 days. Survival rates were compared between these groups. MEASUREMENTS AND MAIN RESULTS:The plasma IL-10 concentrations were increased in control 2, group 2, and group 3 but not in control 1, control 3, or group 1, indicating successful adenovirus gene transfer. Plasma TNF values were significantly reduced in groups 2 and 3 as compared with group 1, with no significant differences in endotoxin concentrations. Survival rates were significantly better in groups 2 and 3 than in group 1 (p < .05). CONCLUSION:These findings suggested that IL-10 has a favorable effect on survival of septic mice via inhibition of TNF production or endotoxin stimulation. |
| 巻・号 | 28(8) |
| ページ | 2685-9 |
| 公開日 | 2000-8-1 |
| DOI | 10.1097/00003246-200008000-00001 |
| PMID | 10966236 |
| MeSH | Adenoviridae / genetics* Animals Escherichia coli / genetics* Gene Transfer Techniques* Interleukin-10 / blood* Interleukin-10 / genetics* Male Mice Mice, Inbred C57BL Prospective Studies Random Allocation Sepsis / blood* Sepsis / mortality* Survival Rate Tumor Necrosis Factor-alpha / analysis |
| IF | 7.414 |
| 引用数 | 13 |
| WOS 分野 | CRITICAL CARE MEDICINE |
| リソース情報 | |
| 遺伝子材料 | AxCAmIL10 Adex1CAmIL10 (RDB1410) |