RRC ID 12221
著者 Isobe K, Yukimasa N, Nakai T, Takuwa Y.
タイトル Pituitary adenylate cyclase-activating polypeptide induces gene expression of the catecholamine synthesizing enzymes, tyrosine hydroxylase and dopamine beta hydroxylase, through 3',5'-cyclic adenosine monophosphate- and protein kinase C-dependent mechanisms in cultured porcine adrenal medullary chromaffin cells.
ジャーナル Neuropeptides
Abstract Pituitary adenylate cyclase-activating polypeptide (PACAP)i a potent stimulant of catecholamine secretion, increased catecholamine production in cultured porcine adrenal medullary chromaffin cells. PACAP induced dose-and time-dependent increases in mRNAs for the catecholamine synthesizing enzymes, tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH), with maximal 6- and 4-fold increases occurring at 8-16 h, respectively. The half-maximally and maximally effective PACAP concentrations for stimulation of TH and DBH gene expression were 0.5 and 3 nM, respectively. The TH protein level also showed an increase over the unstimulated basal level at 16-24 h in PACAP-stimulate cells. We previously demonstrated that PACAP activates both phospholipase C and adenylate cyclase in adrenal medullary cells. Addition of forskolin alone induced increases in mRNA expression of both TH and DBH. The phosphodiesterase inhibitor 3- isobutyl-1-methylxanthine potentiated the induction of TH and DBH mRNAs by PACAP. Addition of the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) also caused increases in TH and DBH mRNA levels. In protein kinase C-downregulated cells pretreated with PMA for 24 h, the stimulatory effect of PACAP on TH and DBH gene expression was diminished. These results suggest that cAMP and protein kinase C mediate the PACAP-induced TH and DBH gene expression. Removal of extracellular Ca2+ with EGTA enhanced the PACAP-induced increases in both cellular cAMP and mRNA levels of TH and DBH, suggesting that Ca2+ has an inhibitory effect on the induction of TH and DBH mRNAs. In conclusion, the present study indicates that PACAP coordinately upregulates the gene expression of both TH and DBH by activating the cAMP and protein kinase C signaling pathways, leading to simulation of cate-cholamine synthesis, while Ca2+ negatively regulates TH and DBH gene expression in porcine adrenal medullary cells.
巻・号 30(2)
ページ 167-75
公開日 1996-4-1
DOI 10.1016/s0143-4179(96)90084-0
PII S0143-4179(96)90084-0
PMID 8771559
MeSH Adrenal Medulla / drug effects Adrenal Medulla / metabolism* Animals Calcium / pharmacology Cells, Cultured Chromaffin System / drug effects Chromaffin System / metabolism Cyclic AMP / metabolism Cyclic AMP / pharmacology* Dopamine beta-Hydroxylase / genetics* Gene Expression / drug effects Neuropeptides / pharmacology* Pituitary Adenylate Cyclase-Activating Polypeptide Protein Kinase C / metabolism* RNA, Messenger / metabolism Swine Tyrosine 3-Monooxygenase / genetics*
IF 2.411
引用数 39
WOS 分野 ENDOCRINOLOGY & METABOLISM NEUROSCIENCES
リソース情報
遺伝子材料 pDBH-1 (RDB01271) pHTH1 (RDB01269)