RRC ID 12716
著者 Kobayashi K, Kikuno I, Kuroha K, Saito K, Ito K, Ishitani R, Inada T, Nureki O.
タイトル Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex.
ジャーナル Proc Natl Acad Sci U S A
Abstract No-go decay and nonstop decay are mRNA surveillance pathways that detect translational stalling and degrade the underlying mRNA, allowing the correct translation of the genetic code. In eukaryotes, the protein complex of Pelota (yeast Dom34) and Hbs1 translational GTPase recognizes the stalled ribosome containing the defective mRNA. Recently, we found that archaeal Pelota (aPelota) associates with archaeal elongation factor 1α (aEF1α) to act in the mRNA surveillance pathway, which accounts for the lack of an Hbs1 ortholog in archaea. Here we present the complex structure of aPelota and GTP-bound aEF1α determined at 2.3-Å resolution. The structure reveals how GTP-bound aEF1α recognizes aPelota and how aPelota in turn stabilizes the GTP form of aEF1α. Combined with the functional analysis in yeast, the present results provide structural insights into the molecular interaction between eukaryotic Pelota and Hbs1. Strikingly, the aPelota·aEF1α complex structurally resembles the tRNA·EF-Tu complex bound to the ribosome. Our findings suggest that the molecular mimicry of tRNA in the distorted "A/T state" conformation by Pelota enables the complex to efficiently detect and enter the empty A site of the stalled ribosome.
巻・号 107(41)
ページ 17575-9
公開日 2010-10-12
DOI 10.1073/pnas.1009598107
PII 1009598107
PMID 20876129
PMC PMC2955123
MeSH Aeropyrum / chemistry* Crystallography, X-Ray Guanosine Triphosphate / metabolism Models, Molecular* Molecular Mimicry Peptide Elongation Factor 1 / chemistry* Peptide Elongation Factor 1 / metabolism Peptide Termination Factors / chemistry* Peptide Termination Factors / metabolism Protein Biosynthesis / genetics Protein Biosynthesis / physiology* Protein Conformation* RNA Stability / physiology RNA, Messenger / chemistry* RNA, Transfer / metabolism Ribosomes / metabolism Yeasts
IF 9.412
引用数 77
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 Aeropyrum pernix JCM 9820T (JGD07499)
一般微生物 JCM 9820