RRC ID 12717
著者 Saito K, Kobayashi K, Wada M, Kikuno I, Takusagawa A, Mochizuki M, Uchiumi T, Ishitani R, Nureki O, Ito K.
タイトル Omnipotent role of archaeal elongation factor 1 alpha (EF1α in translational elongation and termination, and quality control of protein synthesis.
ジャーナル Proc Natl Acad Sci U S A
Abstract The molecular mechanisms of translation termination and mRNA surveillance in archaea remain unclear. In eukaryotes, eRF3 and HBS1, which are homologous to the tRNA carrier GTPase EF1α, respectively bind eRF1 and Pelota to decipher stop codons or to facilitate mRNA surveillance. However, genome-wide searches of archaea have failed to detect any orthologs to both GTPases. Here, we report the crystal structure of aRF1 from an archaeon, Aeropyrum pernix, and present strong evidence that the authentic archaeal EF1α acts as a carrier GTPase for aRF1 and for aPelota. The binding interface residues between aRF1 and aEF1α predicted from aRF1·aEF1α·GTP ternary structure model were confirmed by in vivo functional assays. The aRF1/eRF1 structural domain with GGQ motif, which corresponds to the CCA arm of tRNA, contacts with all three structural domains of aEF1α showing striking tRNA mimicry of aRF1/eRF1 and its GTPase-mediated catalysis for stop codon decoding. The multiple binding capacity of archaeal EF1α explains the absence of GTPase orthologs for eRF3 and HBS1 in archaea species and suggests that universal molecular mechanisms underlie translational elongation and termination, and mRNA surveillance pathways.
巻・号 107(45)
ページ 19242-7
公開日 2010-11-9
DOI 10.1073/pnas.1009599107
PII 1009599107
PMID 20974926
PMC PMC2984191
MeSH Archaeal Proteins / chemistry* Archaeal Proteins / physiology Binding Sites Crystallography, X-Ray GTP Phosphohydrolases / metabolism Molecular Mimicry Peptide Chain Elongation, Translational Peptide Chain Termination, Translational Peptide Elongation Factor 1 / chemistry* Peptide Elongation Factor 1 / physiology Protein Binding Protein Biosynthesis* Protein Conformation RNA, Transfer
IF 9.412
引用数 40
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 Aeropyrum pernix JCM 9820T (JGD07499)
一般微生物 JCM 9820