| RRC ID |
12727
|
| 著者 |
Ohashi Y, Tsubota T, Sato A, Koyano KW, Tamura K, Miyashita Y.
|
| タイトル |
A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits.
|
| ジャーナル |
Mol Cell Neurosci
|
| Abstract |
We developed a bicistronic HIV1-derived lentiviral vector system co-expressing green fluorescent protein (AcGFP1) and wheat germ agglutinin (WGA) mediated by picornaviral 2A peptide. This system was first applied to the analysis of the rat cerebellar efferent pathways. When the lentiviral vector was injected into a specific lobule, the local Purkinje cell population (first-order neurons) was efficiently infected and co-expressed both AcGFP1 and WGA protein. In the second-order neurons in the cerebellar and vestibular nuclei, WGA but not AcGFP1 protein was differentially detected, demonstrating that the presence of AcGFP1 protein enables discrimination of first-order neurons from second-order neurons. Furthermore, WGA protein was detected in the contralateral ventrolateral thalamic nucleus (third-order nucleus). This system also successfully labeled rat cortical pathways from the primary somatosensory cortex and monkey cerebellar efferent pathways. Thus, this bicistronic lentiviral vector system is a useful tool for differential transsynaptic tracing of neural pathways originating from local brain regions.
|
| 巻・号 |
46(1)
|
| ページ |
136-47
|
| 公開日 |
2011-1-1
|
| DOI |
10.1016/j.mcn.2010.08.013
|
| PII |
S1044-7431(10)00204-6
|
| PMID |
20816792
|
| MeSH |
Animals
Cells, Cultured
Cerebellum / anatomy & histology
Gene Transfer Techniques*
Genetic Vectors* / genetics
Genetic Vectors* / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Lentivirus* / genetics
Lentivirus* / metabolism
Macaca
Male
Neural Pathways / anatomy & histology*
Neural Pathways / metabolism
Neurons / metabolism
Neurons / ultrastructure*
Rats
Rats, Wistar
Staining and Labeling / methods*
Synapses / physiology
Synapses / ultrastructure*
Wheat Germ Agglutinins / genetics
Wheat Germ Agglutinins / metabolism
|
| IF |
3.182
|
| 引用数 |
12
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 遺伝子材料 |
CS-CA-MCS (RDB05963) CS-CDF-CG-PRE (RDB04379) |
