RRC ID 15287
著者 Chen HM, Rosebrock AP, Khan SR, Futcher B, Leatherwood JK.
タイトル Repression of meiotic genes by antisense transcription and by Fkh2 transcription factor in Schizosaccharomyces pombe.
ジャーナル PLoS One
Abstract In S. pombe, about 5% of genes are meiosis-specific and accumulate little or no mRNA during vegetative growth. Here we use Affymetrix tiling arrays to characterize transcripts in vegetative and meiotic cells. In vegetative cells, many meiotic genes, especially those induced in mid-meiosis, have abundant antisense transcripts. Disruption of the antisense transcription of three of these mid-meiotic genes allowed vegetative sense transcription. These results suggest that antisense transcription represses sense transcription of meiotic genes in vegetative cells. Although the mechanism(s) of antisense mediated transcription repression need to be further explored, our data indicates that RNAi machinery is not required for repression. Previously, we and others used non-strand specific methods to study splicing regulation of meiotic genes and concluded that 28 mid-meiotic genes are spliced only in meiosis. We now demonstrate that the "unspliced" signal in vegetative cells comes from the antisense RNA, not from unspliced sense RNA, and we argue against the idea that splicing regulates these mid-meiotic genes. Most of these mid-meiotic genes are induced in mid-meiosis by the forkhead transcription factor Mei4. Interestingly, deletion of a different forkhead transcription factor, Fkh2, allows low levels of sense expression of some mid-meiotic genes in vegetative cells. We propose that vegetative expression of mid-meiotic genes is repressed at least two independent ways: antisense transcription and Fkh2 repression.
巻・号 7(1)
ページ e29917
公開日 2012-1-1
DOI 10.1371/journal.pone.0029917
PII PONE-D-11-16845
PMID 22238674
PMC PMC3253116
MeSH Base Sequence Cluster Analysis Down-Regulation / drug effects Gene Expression Profiling Gene Expression Regulation, Fungal / drug effects Genes, Fungal* / drug effects Genes, Fungal* / genetics Meiosis / drug effects Meiosis / genetics* Microarray Analysis Molecular Sequence Data Oligodeoxyribonucleotides, Antisense / pharmacology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* Transcription Factors / genetics Transcription Factors / physiology* Transcription, Genetic / drug effects
IF 2.74
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY16057