RRC ID 15294
著者 Moriya H, Chino A, Kapuy O, Csikász-Nagy A, Novák B.
タイトル Overexpression limits of fission yeast cell-cycle regulators in vivo and in silico.
ジャーナル Mol Syst Biol
Abstract Cellular systems are generally robust against fluctuations of intracellular parameters such as gene expression level. However, little is known about expression limits of genes required to halt cellular systems. In this study, using the fission yeast Schizosaccharomyces pombe, we developed a genetic 'tug-of-war' (gTOW) method to assess the overexpression limit of certain genes. Using gTOW, we determined copy number limits for 31 cell-cycle regulators; the limits varied from 1 to >100. Comparison with orthologs of the budding yeast Saccharomyces cerevisiae suggested the presence of a conserved fragile core in the eukaryotic cell cycle. Robustness profiles of networks regulating cytokinesis in both yeasts (septation-initiation network (SIN) and mitotic exit network (MEN)) were quite different, probably reflecting differences in their physiologic functions. Fragility in the regulation of GTPase spg1 was due to dosage imbalance against GTPase-activating protein (GAP) byr4. Using the gTOW data, we modified a mathematical model and successfully reproduced the robustness of the S. pombe cell cycle with the model.
巻・号 7
ページ 556
公開日 2011-12-6
DOI 10.1038/msb.2011.91
PII msb201191
PMID 22146300
PMC PMC3737731
MeSH Cell Cycle / genetics* Cell Cycle Proteins / genetics Computer Simulation Cytokinesis / genetics GTP Phosphohydrolases / genetics Gene Dosage Gene Expression Regulation, Fungal* Models, Genetic* Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics Systems Biology
IF 8.991
引用数 12
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY7652