論文 - 詳細
| RRC ID | 17482 |
|---|---|
| 著者 | Pathmanathan S, Barnard E, Timson DJ. |
| タイトル | Interactions between the budding yeast IQGAP homologue Iqg1p and its targets revealed by a split-EGFP bimolecular fluorescence complementation assay. |
| ジャーナル | Cell Biol Int |
| Abstract |
A split-EGFP based bimolecular fluorescence complementation (BiFC) assay has been used to detect interactions between the Saccharomyces cerevisiae cytoskeletal scaffolding protein Iqg1p and three targets: myosin essential light chain (Mlc1p), calmodulin (Cmd1p) and the small GTPase Cdc42p. The format of the BiFC assay used ensures that the proteins are expressed at wild type levels thereby avoiding artefacts due to overexpression. This is the first direct in vivo detection of these interactions; in each case, the complex is localised to discrete regions of the yeast cytoplasm. The labelling with EGFP fragments results in changes in growth kinetics, cell size and budding frequency. This is partly due to the reassembled EGFP locking the complexes into essentially permanent interactions. The consequences of this for Iqg1p interactions and BiFC assays in general are discussed. |
| 巻・号 | 32(10) |
| ページ | 1318-22 |
| 公開日 | 2008-10-1 |
| DOI | 10.1016/j.cellbi.2008.07.008 |
| PII | S1065-6995(08)00494-0 |
| PMID | 18675924 |
| MeSH | Biological Assay / methods* Calmodulin / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / metabolism ras GTPase-Activating Proteins / genetics ras GTPase-Activating Proteins / metabolism* |
| IF | 2.571 |
| 引用数 | 5 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 酵母 | BYP6335 BYP6336 |