RRC ID 1904
著者 Ichikawa K, Eki T.
タイトル A novel yeast-based reporter assay system for the sensitive detection of genotoxic agents mediated by a DNA damage-inducible LexA-GAL4 protein.
ジャーナル J Biochem
Abstract Yeast-based genotoxicity testing systems can sensitively detect DNA damaging agents in the environment. We have developed a novel "indirect" reporter assay system based on a recombinant yeast containing both a sensor and a reporter plasmid. The sensor plasmid contains a gene encoding the artificial transcription factor of the Escherichia coli LexA DNA binding domain fused to the transcriptional activation domain of yeast Gal4p, which is regulated by the DNA damage-inducible RNR2 promoter. The reporter plasmid contains the E. coli lacZ gene with the LexA binding site in the 5'-upstream region, allowing transcriptional activation by the induced LexA-GAL4 protein. The activity of DNA damage-dependent beta-galactosidase (beta-gal) in the "indirect" reporter assay system was compared with that of a current yeast-based "direct" reporter system. The "indirect" system exhibited 1.5- to 5-fold greater beta-gal activity upon induction by alkylating agents or camptothecin. To increase the sensitivity of the new reporter system further, several deletion yeast strains were tested, and enhanced induction of reporter activity was observed in DNA repair-deficient mag1Delta cells. The "indirect" 96-well microtiter plate assay system is a potentially inexpensive and sensitive method for detecting genotoxic activities in a wide range of compounds, and in polluted environmental samples.
巻・号 139(1)
ページ 105-12
公開日 2006-1-1
DOI 10.1093/jb/mvj011
PII 139/1/105
PMID 16428325
MeSH Bacterial Proteins / genetics* Base Sequence DNA Damage* DNA Primers DNA-Binding Proteins Genes, Reporter* Mutagenicity Tests Mutagens / analysis Mutagens / toxicity* Saccharomyces cerevisiae Proteins / genetics* Sensitivity and Specificity Serine Endopeptidases / genetics* Transcription Factors / genetics*
IF 2.476
引用数 15
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pGMT20 (RDB01960)