| RRC ID |
2061
|
| 著者 |
Kanatsu-Shinohara M, Ogura A, Ikegawa M, Inoue K, Ogonuki N, Tashiro K, Toyokuni S, Honjo T, Shinohara T.
|
| タイトル |
Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Sertoli cells play a pivotal role in spermatogenesis through their interactions with germ cells. To set up a strategy for treating male infertility caused by Sertoli cell dysfunction, we developed a Sertoli cell gene transfer system by using an adenovirus vector, which maintained long-term transgene expression in the testes of infertile mice. Introduction of an adenovirus carrying the mouse Steel (Sl) gene into Sertoli cells restored partial spermatogenesis in infertile Steel/Steel(dickie) (Sl/Sl(d)) mutant mouse testes. Although these males remained infertile, round spermatids and spermatozoa from the testes produced normal fertile offspring after intracytoplasmic injection into oocytes. None of the offspring showed evidence of germ line transmission of adenoviral DNA. Thus, we demonstrate a successful treatment for infertility by using a gene therapy vector. Therefore, adenovirus-mediated gene delivery into Sertoli cells not only provides an efficient and convenient means for studying germ cell-Sertoli cell interactions through manipulation of the germ cell microenvironment in vivo, but also is a useful method to treat male infertility resulting from a Sertoli cell defect.
|
| 巻・号 |
99(3)
|
| ページ |
1383-8
|
| 公開日 |
2002-2-5
|
| DOI |
10.1073/pnas.022646399
|
| PII |
022646399
|
| PMID |
11818536
|
| PMC |
PMC122199
|
| MeSH |
Adenoviridae / genetics*
Animals
Chromatin / ultrastructure
Crosses, Genetic
Gene Transfer Techniques*
Genetic Vectors*
Infertility, Male / genetics*
Insemination / genetics
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Microinjections
Spermatids / cytology
Spermatogenesis
Spermatozoa / cytology
beta-Galactosidase / genetics
|
| IF |
9.412
|
| 引用数 |
55
|
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
AxCALacZ (RDB1745) |
