RRC ID 2223
著者 Takada H, Nishimura M, Asayama Y, Mannse Y, Ishiwata S, Kita A, Doi A, Nishida A, Kai N, Moriuchi S, Tohda H, Giga-Hama Y, Kuno T, Sugiura R.
タイトル Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast.
ジャーナル Mol Biol Cell
Abstract In fission yeast, knockout of the calcineurin gene resulted in hypersensitivity to Cl(-), and the overexpression of pmp1(+) encoding a dual-specificity phosphatase for Pmk1 mitogen-activated protein kinase (MAPK) or the knockout of the components of the Pmk1 pathway complemented the Cl(-) hypersensitivity of calcineurin deletion. Here, we showed that the overexpression of ptc1(+) and ptc3(+), both encoding type 2C protein phosphatase (PP2C), previously known to inactivate the Wis1-Spc1-Atf1 stress-activated MAPK signaling pathway, suppressed the Cl(-) hypersensitivity of calcineurin deletion. We also demonstrated that the mRNA levels of these two PP2Cs and pyp2(+), another negative regulator of Spc1, are dependent on Pmk1. Notably, the deletion of Atf1, but not that of Spc1, displayed hypersensitivity to the cell wall-damaging agents and also suppressed the Cl(-) hypersensitivity of calcineurin deletion, both of which are characteristic phenotypes shared by the mutation of the components of the Pmk1 MAPK pathway. Moreover, micafungin treatment induced Pmk1 hyperactivation that resulted in Atf1 hyperphosphorylation. Together, our results suggest that PP2C is involved in a negative feedback loop of the Pmk1 signaling, and results also demonstrate that Atf1 is a key component of the cell integrity signaling downstream of Pmk1 MAPK.
巻・号 18(12)
ページ 4794-802
公開日 2007-12-1
DOI 10.1091/mbc.e07-03-0282
PII E07-03-0282
PMID 17881729
PMC PMC2096581
MeSH Activating Transcription Factor 1 / genetics Activating Transcription Factor 1 / metabolism* Calcineurin / deficiency Calcineurin / genetics Calcineurin / metabolism Cell Wall / enzymology Cell Wall / physiology* Gene Dosage / genetics Gene Expression Regulation, Fungal MAP Kinase Signaling System Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / metabolism* Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism* Phosphorylation Protein Phosphatase 2C RNA, Messenger / genetics Schizosaccharomyces / cytology* Schizosaccharomyces / enzymology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Up-Regulation
IF 3.791
引用数 37
WOS 分野 CELL BIOLOGY
リソース情報
酵母 HM123 FY17634(KP898) FY17635(KP907) FY17636(KP909) FY17637(KP1378) FY14017 FY14018