| RRC ID |
22709
|
| 著者 |
Mishra M, Kashiwazaki J, Takagi T, Srinivasan R, Huang Y, Balasubramanian MK, Mabuchi I.
|
| タイトル |
In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics.
|
| ジャーナル |
Nat Cell Biol
|
| Abstract |
Cytokinesis in many eukaryotes involves the contraction of an actomyosin-based contractile ring. However, the detailed mechanism of contractile ring contraction is not fully understood. Here, we establish an experimental system to study contraction of the ring to completion in vitro. We show that the contractile ring of permeabilized fission yeast cells undergoes rapid contraction in an ATP- and myosin-II-dependent manner in the absence of other cytoplasmic constituents. Surprisingly, neither actin polymerization nor its disassembly is required for contraction of the contractile ring, although addition of exogenous actin-crosslinking proteins blocks ring contraction. Using contractile rings generated from fission yeast cytokinesis mutants, we show that not all proteins required for assembly of the ring are required for its contraction in vitro. Our work provides the beginnings of the definition of a minimal contraction-competent cytokinetic ring apparatus.
|
| 巻・号 |
15(7)
|
| ページ |
853-9
|
| 公開日 |
2013-7-1
|
| DOI |
10.1038/ncb2781
|
| PII |
ncb2781
|
| PMID |
23770677
|
| MeSH |
Actins / metabolism*
Adenosine Triphosphate / metabolism
Cell Division
Cell Membrane / metabolism
Cytokinesis / physiology*
Green Fluorescent Proteins / metabolism
Myosin Type II / metabolism*
Schizosaccharomyces / cytology*
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / metabolism*
|
| IF |
20.042
|
| 引用数 |
57
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 酵母 |
FY14500
FY14718
FY14768
FY14771
FY14781
FY14793
FY14812
FY14933
FY14947
FY14957
FY14977
FY15014
FY15055
FY15061
FY15065
FY15226
FY15403
FY15505
FY15507
FY15526
FY17671(MT663)
FY20237(MY6294)
FY13991(YM012)
FY21484(YO3) |
