RRC ID |
2273
|
著者 |
Sakakima Y, Hayashi S, Yagi Y, Hayakawa A, Tachibana K, Nakao A.
|
タイトル |
Gene therapy for hepatocellular carcinoma using sonoporation enhanced by contrast agents.
|
ジャーナル |
Cancer Gene Ther
|
Abstract |
We examined whether sonoporation enhanced by a contrast agent (BR14) was effective in gene therapy for hepatocelluar carcinoma (HCC). Human hepatic cancer cells (SK-Hep1) and plasmid cDNAs expressing green fluorescent protein (GFP), interferonbeta (IFNbeta), and LacZ were used. In vitro, SK-Hep1 cell suspensions with DNA and BR14 were sonoporated. Expressions of every plasmid cDNA and the antitumor effect of IFNbeta were analyzed. In vivo, GFP and IFNbeta genes with BR14 were directly injected into subcutaneous tumors using SK-Hep1 in nude mice, and transcutaneous sonoporation of the tumors was performed. GFP gene transfections and tumor diameters after IFNbeta gene transfection were examined. In vitro, no SK-Hep1 cells were transfected without sonication, whereas transfections were successful after sonication with BR14. Antitumor effect of IFNbeta gene transfection by ultrasound (US) and with BR14 was revealed. In vivo, the SK-Hep1 cells expressed GFP, and the IFNbeta gene transfection by US with BR14 reduced tumor size significantly. In conclusion, gene therapy with sonoporation enhanced by a contrast agent may become a new treatment option for HCC.
|
巻・号 |
12(11)
|
ページ |
884-9
|
公開日 |
2005-11-1
|
DOI |
10.1038/sj.cgt.7700850
|
PII |
7700850
|
PMID |
15891773
|
MeSH |
Animals
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / therapy*
DNA, Complementary / analysis
Enzyme-Linked Immunosorbent Assay
Female
Fluorocarbons
Genetic Therapy / methods*
Green Fluorescent Proteins / analysis
Humans
Interferon-beta / analysis
Liver Neoplasms / genetics
Liver Neoplasms / therapy*
Mice
Mice, Inbred BALB C
Mice, Nude
Phospholipids
Plasmids
Transfection / methods*
Tumor Cells, Cultured
Ultrasonics*
|
IF |
4.534
|
引用数 |
62
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
ONCOLOGY
|
リソース情報 |
遺伝子材料 |
pCMV (Hu beta) (RDB01681)
pCAZ 2 (RDB01870). |