| RRC ID |
27317
|
| 著者 |
Hirai T, Kim YW, Kato K, Hiwasa-Tanase K, Ezura H.
|
| タイトル |
Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter.
|
| ジャーナル |
Transgenic Res
|
| Abstract |
The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.
|
| 巻・号 |
20(6)
|
| ページ |
1285-92
|
| 公開日 |
2011-12-1
|
| DOI |
10.1007/s11248-011-9495-9
|
| PMID |
21359850
|
| MeSH |
Agrobacterium / genetics
Agrobacterium / metabolism
Caulimovirus / genetics
Caulimovirus / metabolism
Fruit / genetics
Fruit / metabolism*
Gene Expression Regulation, Plant*
Genetic Vectors / genetics
Genetic Vectors / metabolism
Glycoproteins / genetics
Glycoproteins / metabolism*
Lycopersicon esculentum / genetics*
Lycopersicon esculentum / metabolism
Pigmentation
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Promoter Regions, Genetic*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Synsepalum / genetics
Transformation, Genetic
|
| IF |
1.856
|
| 引用数 |
8
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| トマト |
TOMJPF00001 |
