| RRC ID |
27714
|
| 著者 |
Kitajima K, Oki S, Ohkawa Y, Sumi T, Meno C.
|
| タイトル |
Wnt signaling regulates left-right axis formation in the node of mouse embryos.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The node triggers formation of the left-right axis in mouse embryos by establishing local asymmetry of Nodal and Cerl2 expression. We found that Wnt3 is expressed in perinodal crown cells preferentially on the left side. The enhancer responsible for Wnt3 expression was identified and found to be regulated by Foxa2 and Rbpj under the control of Notch signaling. Rbpj binding sites suppress enhancer activity in pit cells of the node, thereby ensuring crown cell-specific expression. In addition, we found that the expression of Gdf1 and Cerl2 is also regulated by Notch signaling, suggesting that such signaling may induce the expression of genes related to left-right asymmetry as a set. Furthermore, Cerl2 expression became symmetric in response to inhibition of Wnt-β-catenin signaling. Our results suggest that Wnt signaling regulates the asymmetry of Cerl2 expression, which likely generates a left-right difference in Nodal activity at the node for further amplification in lateral plate mesoderm.
|
| 巻・号 |
380(2)
|
| ページ |
222-32
|
| 公開日 |
2013-8-15
|
| DOI |
10.1016/j.ydbio.2013.05.011
|
| PII |
S0012-1606(13)00255-8
|
| PMID |
23707899
|
| MeSH |
Animals
Body Patterning*
Female
Hepatocyte Nuclear Factor 3-beta / physiology
Immunoglobulin J Recombination Signal Sequence-Binding Protein / physiology
Intercellular Signaling Peptides and Proteins / physiology
Mice
Mice, Inbred ICR
Wnt Signaling Pathway / physiology*
Wnt3 Protein / physiology
|
| IF |
2.896
|
| 引用数 |
19
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pEF-BOSneo-mNotch1 RAMIC (RDB06771) |
