RRC ID 27764
著者 Yi C, Zhang W, Dai X, Li X, Gong Z, Zhou Y, Liang G, Gu M.
タイトル Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha.
ジャーナル Chromosome Res
Abstract The centromere is a key chromosomal component for sister chromatid cohesion and is the site for kinetochore assembly and spindle fiber attachment, allowing each sister chromatid to faithfully segregate to each daughter cell during cell division. It is not clear what types of sequences act as functional centromeres and how centromere sequences are organized in Oryza brachyantha, an FF genome species. In this study, we found that the three classes of centromere-specific CentO-F satellites (CentO-F1, CentO-F2, and CentOF3) in O. brachyantha share no homology with the CentO satellites in Oryza sativa. The three classes of CentO-F satellites are all located within the chromosomal regions to which the spindle fibers attach and are characterized by megabase tandem arrays that are flanked by centromere-specific retrotransposons, CRR-F, in the O. brachyantha centromeres. Although these CentO-F satellites are quantitatively variable among 12 O. brachyantha centromeres, immunostaining with an antibody specific to CENH3 indicates that they are colocated with CENH3 in functional centromere regions. Our results demonstrate that the three classes of CentO-F satellites may be the major components of functional centromeres in O. brachyantha.
巻・号 21(8)
ページ 725-37
公開日 2013-12-1
DOI 10.1007/s10577-013-9374-8
PMID 24077888
MeSH Base Sequence Centromere / genetics* Chromosomes, Plant / genetics DNA, Plant / genetics DNA, Plant / isolation & purification DNA, Satellite / genetics DNA, Satellite / isolation & purification Evolution, Molecular Genetic Variation* Genome, Plant* In Situ Hybridization, Fluorescence Molecular Sequence Data Poaceae / genetics* Retroelements Sequence Alignment Sequence Analysis, DNA Tandem Repeat Sequences
IF 3.413
引用数 7
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
イネ W1057 W1577 W1263 W0008