| RRC ID |
28644
|
| 著者 |
Kurabe N, Katagiri K, Komiya Y, Ito R, Sugiyama A, Kawasaki Y, Tashiro F.
|
| タイトル |
Deregulated expression of a novel component of TFTC/STAGA histone acetyltransferase complexes, rat SGF29, in hepatocellular carcinoma: possible implication for the oncogenic potential of c-Myc.
|
| ジャーナル |
Oncogene
|
| Abstract |
c-Myc N-terminal conserved domains, MbI and MbII, are essential for c-Myc-mediated transformation and transactivation. These domains recruit the STAGA (SPT3-TAF9-GCN5-acetyltransferase) coactivator complex, but not TFTC (TATA-binding protein-free TAF-containing) to the target gene promoter. Although components of this complex are well conserved between yeast and mammals, four mammalian orthologs of yeast SPT8, SPT20, SGF11 and SGF29 remain to be identified. Here, we isolated a rat ortholog of yeast SGF29, a component of yeast SAGA (SPT-ADA-GCN5-acetyltransferase) complex. Both rat (r) SGF29 and c-myc mRNAs were overexpressed in five out of the eight tested rodent tumor cells. rSGF29 directly interacted with rADA3 and co-immunoprecipitated with two other TFTC/STAGA components, rGCN5 and rSPT3. rSGF29 was recruited to the c-Myc target gene promoters together with c-Myc, and it activated c-Myc target gene expressions. Downregulation of rSGF29 suppressed the expression of c-Myc target genes and inhibited anchorage-independent growth and tumorigenicity and lung metastasis of rat hepatoma K2 cells when injected into nude mice. These results show that rSGF29 is a novel component of TFTC/STAGA complexes and could be involved in the c-Myc-mediated malignant transformation.
|
| 巻・号 |
26(38)
|
| ページ |
5626-34
|
| 公開日 |
2007-8-16
|
| DOI |
10.1038/sj.onc.1210349
|
| PII |
1210349
|
| PMID |
17334388
|
| MeSH |
Animals
Blotting, Northern
Blotting, Western
COS Cells
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / pathology*
Cell Line, Tumor
Chlorocebus aethiops
Gene Expression Regulation
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Histone Acetyltransferases / genetics
Histone Acetyltransferases / metabolism*
Liver Neoplasms, Experimental / genetics
Liver Neoplasms, Experimental / metabolism
Liver Neoplasms, Experimental / pathology*
Mice
Mice, Nude
Oligonucleotides, Antisense / genetics
Protein Subunits / genetics
Protein Subunits / metabolism
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism*
Rats
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Transcriptional Activation
Transfection
Tumor Burden
Two-Hybrid System Techniques
|
| IF |
7.971
|
| 引用数 |
20
|
|
WOS 分野
|
GENETICS & HEREDITY
ONCOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pcDNA3/HA-rSGF29 (RDB07944) |
