RRC ID 28700
著者 Dorywalska M, Coukell B, Dharamsi A.
タイトル Characterization and hetereologous expression of cDNAs encoding two novel closely related Ca(2+)-binding proteins in Dictyostelium discoideum.
ジャーナル Biochim Biophys Acta
Abstract During a yeast two hybrid screen of a Dictyostelium cDNA library using the Ca(2+)-binding protein CBP1 as bait, we isolated a full-length cDNA encoding a novel Ca(2+)-binding protein (termed CBP4a). The protein is composed of 162 amino acids and contains four consensus EF-hands. PCR amplification of Dictyostelium genomic DNA using primers specific for the cDNA sequence resulted in the isolation of a gene encoding a different Ca(2+)-binding protein of 162 amino acids (designated CBP4b) with 90% amino acid sequence identity to CBP4a. Southern blot analysis confirmed the presence of two closely related genes in the Dictyostelium genome. CBP4a and CBP4b mRNAs are expressed at the same stages of development as CBP1 mRNA. In addition, both novel proteins bind (45)Ca(2+) and interact with CBP1 in vitro in a Ca(2+)-dependent manner.
巻・号 1496(2-3)
ページ 356-61
公開日 2000-4-17
DOI 10.1016/s0167-4889(00)00024-0
PII S0167-4889(00)00024-0
PMID 10771103
MeSH Amino Acid Sequence Animals Calcium-Binding Proteins / chemistry Calcium-Binding Proteins / genetics* DNA, Complementary / chemistry DNA, Complementary / isolation & purification Dictyostelium / genetics* Dictyostelium / growth & development Dictyostelium / metabolism Gene Library Genes, Protozoan* Molecular Sequence Data RNA, Messenger / analysis Sequence Alignment
IF 3.411
引用数 14
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
細胞性粘菌 G02100