RRC ID 28743
著者 Nakano A, Masuda K, Hiromoto T, Takahashi K, Matsumoto Y, Habib AG, Darwish AG, Yukawa M, Tsuchiya E, Ueno M.
タイトル Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.
ジャーナル Mol Cell Biol
Abstract The spindle assembly checkpoint (SAC) monitors defects in kinetochore-microtubule attachment or lack of tension at kinetochores and arrests cells at prometaphase. In fission yeast, the double mutant between pot1Δ and the helicase-dead point mutant of the RecQ helicase Rqh1 gene (rqh1-hd) accumulates Rad51-dependent recombination intermediates at telomeres and enters mitosis with those intermediates. Here, we found that SAC-dependent prometaphase arrest occurred more frequently in pot1Δ rqh1-hd double mutants than in rqh1-hd single mutants. SAC-dependent prometaphase arrest also occurred more frequently in rqh1-hd single mutants after cells were released from DNA replication block compared to the rqh1-hd single mutant in the absence of exogenous insult to the DNA. In both cases, Mad2 foci persisted longer than usual at kinetochores, suggesting a defect in kinetochore-microtubule attachment. In pot1Δ rqh1-hd double mutants and rqh1-hd single mutants released from DNA replication block, SAC-dependent prometaphase arrest was suppressed by the removal of the recombination or replication intermediates. Our results indicate that the accumulation of recombination or replication intermediates induces SAC-dependent prometaphase arrest, possibly by affecting kinetochore-microtubule attachment.
巻・号 34(8)
ページ 1389-97
公開日 2014-4-1
DOI 10.1128/MCB.01704-13
PII MCB.01704-13
PMID 24469396
PMC PMC3993586
MeSH Animals Cell Cycle Proteins / genetics Chromosome Segregation / genetics Chromosomes, Fungal / genetics DNA Repair / genetics DNA, Ribosomal / genetics* Genes, cdc / genetics* Kinetochores / metabolism M Phase Cell Cycle Checkpoints / genetics Mitosis / genetics Mitosis / physiology Mutation / genetics Rad51 Recombinase / genetics* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Spindle Apparatus / genetics* Telomere / genetics*
IF 3.611
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 FY18585 FY10134 FY18581 FY18537