論文 - 詳細
| RRC ID | 28940 |
|---|---|
| 著者 | Witke W, Nellen W, Noegel A. |
| タイトル | Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. |
| ジャーナル | EMBO J |
| Abstract |
Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene. |
| 巻・号 | 6(13) |
| ページ | 4143-8 |
| 公開日 | 1987-12-20 |
| DOI | 10.1002/j.1460-2075.1987.tb02760.x |
| PMID | 2832153 |
| PMC | PMC553897 |
| MeSH | Actinin / genetics* DNA Restriction Enzymes Dictyostelium / genetics* Genes* Genes, Fungal* Genetic Vectors RNA, Messenger / genetics* Recombination, Genetic* Transformation, Genetic |
| IF | 9.889 |
| 引用数 | 147 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 細胞性粘菌 | S00416 |