| RRC ID |
28997
|
| 著者 |
Nagasaki A, Sutoh K, Adachi H, Sutoh K.
|
| タイトル |
A novel Dictyostelium discoideum gene required for cAMP-dependent cell aggregation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Using a method of random insertional mutagenesis called REMI (restriction enzyme-mediated integration), we isolated two mutant strains of Dictyostelium discoideum with a defect in cAMP-dependent cell aggregation. On bacterial lawns, both of the cells formed large and smooth plaques. When starved in a non-nutrient medium, they became elongated and extended pseudopods very frequently like starved wild type cells. However, they never formed streams toward an aggregation center. Genomic DNA fragments flanking the sites of insertion of the REMI tag were rescued from the mutant cells. The fragments contained one common open reading frame encoding a protein of 1148 amino acid residues. The protein's sequence is homologous to those of two hypothetical proteins of S. cerevisiae and S. pombe.
|
| 巻・号 |
244(2)
|
| ページ |
505-13
|
| 公開日 |
1998-3-17
|
| DOI |
10.1006/bbrc.1998.8230
|
| PII |
S0006-291X(98)98230-4
|
| PMID |
9514897
|
| MeSH |
Amino Acid Sequence
Animals
Chemotaxis / drug effects
Chemotaxis / genetics
Cloning, Molecular
Cyclic AMP / pharmacology*
Dictyostelium / cytology
Dictyostelium / drug effects*
Dictyostelium / genetics*
Fungal Proteins / genetics
Gene Expression
Gene Targeting
Genes, Fungal*
Genes, Protozoan*
Molecular Sequence Data
Mutation
Phenotype
Protozoan Proteins / genetics
Saccharomyces cerevisiae / genetics
Schizosaccharomyces / genetics
Sequence Homology, Amino Acid
|
| IF |
2.985
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
S00051
S00302
S00309
S00552 |
