RRC ID 29053
著者 Muramoto T, Suzuki K, Shimizu H, Kohara Y, Kohriki E, Obara S, Tanaka Y, Urushihara H.
タイトル Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum.
ジャーナル Mech Dev
Abstract Macrocysts in Dictyostelium discoideum possess prototypic features of sexual reproduction and are useful for understanding the basic mechanisms of the reproductive process. Here, we randomly analyzed 1,071 gamete cDNAs, and then constructed a gamete-specific subtraction library, FC-IC. Nucleotide sequences of all 903 FC-IC clones were determined and clustered into 272 independent genes. Expression analysis based on real-time RT-PCR revealed 67 gamete-enriched genes, among which those involved in 'signal transduction' and 'multicellular organization' are prevalent. One of them, FC-IC0003, appeared also to be mating-type specific, and was named gmsA. RNAi-mediated silencing as well as disruption of gmsA reduced the cellular competency for sexual cell fusion, indicating the involvement of this gene in the sexual development of D. discoideum.
巻・号 120(8)
ページ 965-75
公開日 2003-8-1
DOI 10.1016/s0925-4773(03)00136-9
PII S0925477303001369
PMID 12963116
MeSH Amino Acid Sequence Animals Base Sequence Dictyostelium / genetics* Gene Expression Profiling Gene Library Gene Pool* Germ Cells / physiology* Molecular Sequence Data RNA, Antisense / genetics RNA, Antisense / metabolism* RNA, Small Interfering Sequence Alignment Signal Transduction
IF 2.126
引用数 25
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 S00262 S00388 S90377 G01143 G01144 G01145 G01146 G01147 G01148 G01149 G01150 G01152 G01719 G01720 G01721 G01722 G01723 G01724 G01725 G01726 G01727 G01728 G01729 G01730 G01731 G01732 G01733 G01734 G01735 G01736 G01737 G01738 G01739 G01740 G01741 G01742 G01743 G01744 G01745 G01746 G01747 G01748 G01749 G01750 G01751 G01753 G01754 G01755 G01756 G01757 G01758 G01759 G01760 G01761 G03222 G03223 G03224 G03225 G03226 G03228 G03229 G03230 G03231 G03232 G03233 G03234 G03235 G03236 G03238 G03239 G03240 G03241 G03242 G03243 G03244 G03245 G03246 G03247 G03248 G03249 G03250 G03251 G03252 G03253 G03254 G03255 G03256 G03257 G03258 G03259 G03260 G03261 G03262 G03263 G03264 G03265 G03266 G03267 G03268 G03269 G03270 G03281 G20137 G20523 G20629 G20653 G22556 G22557 G22558 G22559 G24022 G24023 G24024 G24025 G24026 G24027 G24028 G24029 G24030 G24031 G24032 G24033 G24034 G24035 G24036 G24037 G24038 G24039 G24040 G24041 G24042 G24043 G24044 G24045 G24046 G24047 G24048 G24049 G24050 G24051 G24052 G24053 G24054 G24055 G24056 G24057 G24058 G24059 G24060 G24061 G24062 G24063 G24064 G24065 G24066 G24067 G24068 G24069 G24070 G24071 G24072 G24073 G24074 G24075 G24076 G24077 G24078 G24079 G24080 G24081 G24082 G24083 G24084 G24085 G24086 G24087 G24088 G24089 G24090 G24091 G24092 G24093 G24094 G24270 G24271 G24272 G24273 G24274 G24275 G24276 G24277 G24278 G24279 G24280 G24281 G24282 G24283 G24284 G24285 G24286 G24287 G24288 G24289 G24290 G24291 G24292 G24293 G24294 G24295 G24296 G24297 G24298 G24299 G24300 G24301 G24302 G24303 G24304 G24305 G24306 G24307 G24308 G24309 G24310 G24311 G24312 G24313 G24314 G24315 G24316 G24317 G24318 G24319 G24320 G24321 G24322 G24323 G24324 G24517 G24518 G24519 G24520 G24521 G24522 G24523 G24524 G24525 G24526 G24527 G24528 G24529 G24530 G24531 G24532 G24533 G24534 G24535