RRC ID 29080
著者 Clarke M, Müller-Taubenberger A, Anderson KI, Engel U, Gerisch G.
タイトル Mechanically induced actin-mediated rocketing of phagosomes.
ジャーナル Mol Biol Cell
Abstract Actin polymerization can be induced in Dictyostelium by compressing the cells to bring phagosomes filled with large particles into contact with the plasma membrane. Asymmetric actin assembly results in rocketing movement of the phagosomes. We show that the compression-induced assembly of actin at the cytoplasmic face of the plasma membrane involves the Arp2/3 complex. We also identify two other proteins associated with the mechanically induced actin assembly. The class I myosin MyoB accumulates at the plasma membrane-phagosome interface early during the initiation of the response, and coronin is recruited as the actin filaments are disassembling. The forces generated by rocketing phagosomes are sufficient to push the entire microtubule apparatus forward and to dislocate the nucleus.
巻・号 17(11)
ページ 4866-75
公開日 2006-11-1
DOI 10.1091/mbc.e06-04-0365
PII E06-04-0365
PMID 16971511
PMC PMC1635377
MeSH Actin-Related Protein 2-3 Complex / metabolism Actins / metabolism* Animals Cell Membrane / metabolism Cell Nucleus / metabolism Dictyostelium / cytology Dictyostelium / metabolism* Endosomes / metabolism Microtubules / metabolism Nonmuscle Myosin Type IIB / metabolism Phagosomes / metabolism* Protein Transport Recombinant Fusion Proteins / metabolism Stress, Mechanical Time Factors
IF 3.791
引用数 24
WOS 分野 CELL BIOLOGY
リソース情報
細胞性粘菌 S90285