RRC ID 29115
著者 Nagata T, Suzuki Y, Ueno K, Kokubo H, Xu X, Hui C, Hara W, Fukuta M.
タイトル Developmental expression of the Bombyx Antennapedia homologue and homeotic changes in the Nc mutant.
ジャーナル Genes Cells
Abstract BACKGROUND:Abundant availability of homeotic mutants in Bombyx mori provides us with clues to study the body plan of the silkworm. Previous studies indicated that the ECa/ECa and EN/EN embryos, which reveal drastic morphological changes (Itikawa 1943, 1952) lack the homologue of abd-A and the homologues of abd-A and Ubx, respectively (Ueno et al. 1992). It will be interesting to characterize a mutant named Nc, the locus of which was mapped about 1.4 cm apart from the E loci (Itikawa 1944, 1952).
RESULTS:In the present study we cloned and identified the Bombyx Antennapedia cDNA from a library of larval middle silk gland. The developmental expression patterns of the Bombyx Antennapedia were analysed in embryos and larval middle silk glands. We found the Bombyx Antennapedia mRNA of the Nc mutant lacks the homeobox region and its downstream region. This defect comes from a corresponding deletion on the Nc chromosome. Development of the Nc/Nc embryos terminates at stage 26, revealing a transformation of the prothoracic legs into antennae, milder changes in the mesothoracic legs, compression between the prothoracic and mesothoracic segments as well as in the gnathocephalon region, and a severe growth suppression of the silk glands.
CONCLUSIONS:These results give a molecular basis and detailed morphological abnormalities of the Nc mutant known since 1944.
巻・号 1(6)
ページ 555-68
公開日 1996-6-1
DOI 10.1046/j.1365-2443.1996.d01-260.x
解説 Nc(Nc斑紋)の原因遺伝子はBmAntpである。BmAntpは、ショウジョウバエのホメオティック遺伝子であるAntpのカイコホモログである。Ncを持った第6染色体では、BmAntpの3'側が欠失している。
PMID 9078385
MeSH Amino Acid Sequence Animals Antennapedia Homeodomain Protein Base Sequence Bombyx / embryology Bombyx / genetics* Bombyx / growth & development Cloning, Molecular DNA, Complementary / genetics Gene Expression Regulation, Developmental Genes, Homeobox* Genes, Insect* Homeodomain Proteins / genetics* Larva / metabolism Molecular Sequence Data Mutation Nuclear Proteins* Open Reading Frames Phenotype RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Homology, Amino Acid Transcription Factors*
IF 1.655
引用数 20
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
カイコ f39