論文 - 詳細
| RRC ID | 29190 |
|---|---|
| 著者 | Nakańo A, Muramatsu M. |
| タイトル | A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. |
| ジャーナル | J Cell Biol |
| Abstract |
SAR1, a gene that has been isolated as a multicopy suppressor of the yeast ER-Golgi transport mutant sec12, encodes a novel GTP-binding protein. Its nucleotide sequence predicts a 21-kD polypeptide that contains amino acid sequences highly homologous to GTP-binding domains of many ras-related proteins. Gene disruption experiments show that SAR1 is essential for cell growth. To test its function further, SAR1 has been placed under control of the GAL1 promoter and introduced into a haploid cell that had its chromosomal SAR1 copy disrupted. This mutant grows normally in galactose medium but arrests growth 12-15 h after transfer to glucose medium. At the same time, mutant cells accumulate ER precursor forms of a secretory pheromone, alpha-mating factor, and a vacuolar enzyme, carboxypeptidase Y. We propose that Sec12p and Sarlp collaborate in directing ER-Golgi protein transport. |
| 巻・号 | 109(6 Pt 1) |
| ページ | 2677-91 |
| 公開日 | 1989-12-1 |
| DOI | 10.1083/jcb.109.6.2677 |
| PMID | 2512296 |
| PMC | PMC2115904 |
| MeSH | Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular Endoplasmic Reticulum / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism GTP-Binding Proteins / genetics* GTP-Binding Proteins / metabolism Genetic Complementation Test Genotype Golgi Apparatus / metabolism* Information Systems Molecular Sequence Data Nucleic Acid Hybridization Plasmids Protein Processing, Post-Translational* Restriction Mapping Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism Sequence Homology, Nucleic Acid |
| IF | 8.811 |
| 引用数 | 360 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | pANY2-7 (RDB08906) pANY2-9 (RDB08907) pANY2-17 (RDB08908) pANY2-18 (RDB08909) pANY2-19 (RDB08910) pANY3-11 (RDB08911) pANY3-14 (RDB08912) |