RRC ID |
29204
|
著者 |
Nakano A, Otsuka H, Yamagishi M, Yamamoto E, Kimura K, Nishikawa S, Oka T.
|
タイトル |
Mutational analysis of the Sar1 protein, a small GTPase which is essential for vesicular transport from the endoplasmic reticulum.
|
ジャーナル |
J Biochem
|
Abstract |
SAR1 encodes a 21-kDa GTPase, which is required for vesicle formation from the endoplasmic reticulum in yeast. Although it belongs to the expanding small GTPase superfamily, there are interesting structural features that are unique to the Sar1 protein. We performed a site-directed mutational study to identify the amino acid residues that are essential for the Sar1p function. Among seven mutants we constructed, four are functionless by themselves, while two confer temperature sensitivity to cells. When the mutant proteins are overproduced in wild-type cells, all of these six show a dominant negative effect on cell growth. The replacement by serine of the only cysteine residue present in Sar1p caused no significant change in the growth phenotype. These findings are not only important for analyzing the mechanism of the Sar1p action in yeast, but will also be very useful for studying the function of Sar1p counterparts in higher eukaryotes.
|
巻・号 |
116(2)
|
ページ |
243-7
|
公開日 |
1994-8-1
|
DOI |
10.1093/oxfordjournals.jbchem.a124513
|
PMID |
7822237
|
MeSH |
Amino Acid Sequence
Biological Transport, Active
DNA Mutational Analysis
Endoplasmic Reticulum / metabolism*
Fungal Proteins / genetics*
Fungal Proteins / physiology*
GTP-Binding Proteins / genetics*
GTP-Binding Proteins / physiology*
Molecular Sequence Data
Monomeric GTP-Binding Proteins*
Phenotype
Plasmids / genetics
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins*
Transformation, Genetic
Vesicular Transport Proteins
|
IF |
2.476
|
引用数 |
23
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
遺伝子材料 |
pMYY3-1 (RDB08885)
pMYY3-2 (RDB08886)
pMYY3-3 (RDB08887)
pMYY3-4 (RDB08888)
pMYY3-5 (RDB08889)
pMYY3-6 (RDB08890)
pMYY3-7 (RDB08891)
pMYY3-8 (RDB08892)
pMYY3-9 (RDB08893)
pMYY4-1 (RDB08894)
pMYY4-2 (RDB08895)
pMYY4-3 (RDB08896)
pMYY4-4 (RDB08897)
pMYY4-5 (RDB08898)
pMYY4-6 (RDB08899)
pMYY4-7 (RDB08900)
pMYY4-8 (RDB08901)
pMYY4-9 (RDB08902) |