RRC ID 29729
著者 Armstrong GD, Yamazaki H.
タイトル Isolation and characterization of catabolite repression-resistant mutants of Escherichia coli.
ジャーナル Can J Microbiol
Abstract A method has been developed for the isolation of Escherichia coli mutants which are resistant to catabolic repression. The method is based on the fact that a mixture of glucose and gluconate inhibits the development of chemotactic motility in the wild type, but not in the mutants. A motile E. coli strain was mutagenized and grown in glucose and gluconate. Mutants which were able to swim into a tube containing a chemotactic attractant (aspartic acid) were isolated. Most of these mutants were able to produce beta-galactosidase in the presence of glucose and gluconate and were normal in their ability to degrade adenosine 3',5-cyclic monophosphate. Some of these mutants were defective in the glucose phosphotransferase system.
巻・号 23(10)
ページ 1384-93
公開日 1977-10-1
DOI 10.1139/m77-207
PMID 200326
MeSH 3',5'-Cyclic-AMP Phosphodiesterases / metabolism Chemotaxis Chromosome Mapping Chromosomes, Bacterial Enzyme Induction / drug effects Enzyme Repression* Escherichia coli / enzymology Escherichia coli / genetics* Galactosidases / biosynthesis Genes* Gluconates / metabolism Glucose / metabolism Glucose / pharmacology Mutation Tryptophanase / biosynthesis
IF 1.793
リソース情報
原核生物(大腸菌) ME8067