論文 - 詳細
| RRC ID | 29803 |
|---|---|
| 著者 | Singer M, Baker TA, Schnitzler G, Deischel SM, Goel M, Dove W, Jaacks KJ, Grossman AD, Erickson JW, Gross CA. |
| タイトル | A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. |
| ジャーナル | Microbiol Rev |
| Abstract |
We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most positions both Tn10 (Tetr) and TN10kan (Kanr) elements are available, so that the collection contains a linked set of alternating antibiotic resistance markers. The map position of each insertion has been aligned to the E. coli genetic map as well as to the Kohara ordered clone bank. These strains are designed to be used in a rapid two-step mapping system in E. coli. In the first step, the mutation is localized to a 5- to 15-min region of the chromosome by Hfr mapping with a set of Hfr strains containing either Tn10 or Tn10kan elements located 20 min from their respective origins of transfer. In the second step, the mutation is localized to a 1-min region by P1 transduction, with a collection of isogenic insertion strains as donors. We discuss the uses of this collection of strains to map and eventually to clone a variety of mutations in E. coli. |
| 巻・号 | 53(1) |
| ページ | 1-24 |
| 公開日 | 1989-3-1 |
| DOI | 10.1128/mr.53.1.1-24.1989 |
| PMID | 2540407 |
| PMC | PMC372715 |
| MeSH | Chromosome Mapping* Chromosomes, Bacterial* DNA Transposable Elements Drug Resistance, Microbial / genetics Escherichia coli / drug effects Escherichia coli / genetics* Mutation R Factors* Transduction, Genetic |
| 引用数 | 693 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME8979 ME8970 ME8795 ME8796 ME8940 ME8941 ME8944 ME8945 ME8946 ME8947 ME8948 ME8949 ME8950 ME8951 ME8952 ME8971 ME8972 ME8973 ME8974 ME8975 ME8976 ME8978 ME8980 ME8981 ME8982 ME8983 ME8984 ME8953 ME8957 ME8958 ME8959 ME8960 ME8961 ME8962 ME8963 ME8964 ME8965 ME8849 ME8966 ME8968 ME8969 ME8912 ME8913 ME8915 ME8916 ME8917 ME8918 ME8919 ME8920 ME8921 ME8922 ME8923 ME8924 ME8925 ME8926 ME8927 ME8929 ME8930 ME8931 ME8932 ME8934 ME8935 ME8937 ME8938 ME8939 ME8883 ME8884 ME8886 ME8887 ME8889 ME8891 ME8892 ME8893 ME8894 ME8896 ME8897 ME8898 ME8899 ME8900 ME8901 ME8902 ME8903 ME8904 ME8905 ME8906 ME8907 ME8908 ME8910 ME8911 ME8855 ME8856 ME8857 ME8858 ME8859 ME8860 ME8861 ME8862 ME8864 ME8865 ME8867 ME8869 ME8870 ME8871 ME8872 ME8874 ME8875 ME8876 ME8877 ME8878 ME8879 ME8880 ME8881 ME8882 ME8822 ME8823 ME8824 ME8825 ME8826 ME8827 ME8828 ME8829 ME8831 ME8832 ME8833 ME8835 ME8838 ME8839 ME8840 ME8841 ME8842 ME8843 ME8844 ME8845 ME8846 ME8847 ME8848 ME8850 ME8851 ME8854 ME8792 ME8793 ME8794 ME8797 ME8799 ME8801 ME8802 ME8803 ME8804 ME8805 ME8806 ME8807 ME8809 ME8810 ME8811 ME8812 ME8813 ME8814 ME8815 ME8816 ME8817 ME8818 ME8819 ME8820 ME8821 ME8781 ME8782 ME8783 ME8784 ME8785 ME8786 ME8787 ME8788 ME8790 ME8791 ME8873 |