RRC ID 29814
著者 Miwa Y, Fujita Y.
タイトル Purification and characterization of a repressor for the Bacillus subtilis gnt operon.
ジャーナル J Biol Chem
Abstract The GntR protein is a negative regulator involved in gluconate-inducible expression of the Bacillus subtilis gnt operon which is responsible for gluconate metabolism. The GntR protein has been purified to homogeneity from an overproducing Escherichia coli strain harboring a gntR gene-carrying plasmid. The total amino acid composition and the NH2-terminal amino acid sequence of the purified protein were essentially the same as those deduced from the nucleotide sequence of the gntR gene. Molecular weight determination by gel filtration revealed that the purified protein is in a highly polymerized form, but it likely exists as a dimer when highly diluted. The purified GntR protein was found to be specifically bound to DNA fragments carrying the promoter of the gnt operon in an electrophoretic mobility shift assay. This binding was specifically inhibited by the addition of gluconate or glucono-delta-lactone. The purified protein repressed in vitro transcription from the promoter of the gnt operon. This repression was suppressed by gluconate or glucono-delta-lactone. These results indicate that the GntR protein is a repressor for the gnt operon and that gluconate and glucono-delta-lactone are inducers for this operon.
巻・号 263(26)
ページ 13252-7
公開日 1988-9-15
PII S0021-9258(18)37698-1
PMID 2843515
MeSH Bacillus subtilis / genetics* Bacterial Proteins / genetics Bacterial Proteins / isolation & purification* DNA, Bacterial / metabolism Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Gluconates / pharmacology Lactones Molecular Weight Operon* Plasmids Repressor Proteins / isolation & purification* Transcription Factors / isolation & purification* Transcription, Genetic
IF 4.238
引用数 36
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9629