論文 - 詳細
| RRC ID | 29815 |
|---|---|
| 著者 | Parker B, Marinus MG. |
| タイトル | A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. |
| ジャーナル | Gene |
| Abstract |
We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli. |
| 巻・号 | 73(2) |
| ページ | 531-5 |
| 公開日 | 1988-12-20 |
| DOI | 10.1016/0378-1119(88)90517-3 |
| PII | 0378-1119(88)90517-3 |
| PMID | 2854098 |
| MeSH | Chromosome Deletion* Chromosome Mapping Chromosomes, Bacterial Crosses, Genetic DNA Transposable Elements* Escherichia coli / enzymology Escherichia coli / genetics* Genes* Genes, Bacterial* Genotype Mutation* Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics* Transduction, Genetic |
| IF | 2.984 |
| 引用数 | 63 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | ME7815 |