| RRC ID |
29837
|
| 著者 |
Nakayama K, Irino N, Nakayama H.
|
| タイトル |
The recQ gene of Escherichia coli K12: molecular cloning and isolation of insertion mutants.
|
| ジャーナル |
Mol Gen Genet
|
| Abstract |
The recQ gene of Escherichia coli K12 was subcloned from plasmid pKO1 (Oeda et al. 1981) by monitoring the capacity of the resulting recombinant plasmids partially to reverse the increased ultraviolet (UV) sensitivity of a recF143 recQ1 double mutant. We were able to trace this complementation activity to a 3.4 kilobase (kb) SalI-PvuII fragment. Furthermore, analysis of the Tn3 insertion mutations that abolished the complementation revealed the exclusive localisation of such insertions in the same 3.4 kb segment. This segment was situated about 4 kb clockwise from corA on the chromosome, a result consistent with the transductional data previously reported. In addition, a comparison of our restriction endonuclease cleavage map with the published data has placed recQ between pldA and pldB. When relocated to the recQ site on the chromosome, the recQ::Tn3 mutations conferred partial resistance to thymineless death (TLD) or, in the case of a recBC sbcB background, recombination deficiency and increased UV sensitivity. This has provided the firm evidence that both the TLD resistance and the deficiency in the RecF recombination pathway result from loss of the functional recQ gene. We also identified the recQ gene product as a 74 kilodalton polypeptide by using the maxicell technique.
|
| 巻・号 |
200(2)
|
| ページ |
266-71
|
| 公開日 |
1985-1-1
|
| DOI |
10.1007/BF00425434
|
| PMID |
2993821
|
| MeSH |
Cloning, Molecular*
Conjugation, Genetic
DNA Restriction Enzymes
DNA Transposable Elements*
Escherichia coli / genetics*
Escherichia coli / radiation effects
Genes, Bacterial*
Mutation*
Phenotype
Plasmids
Species Specificity
Thymine / metabolism
Transduction, Genetic
Ultraviolet Rays
|
| 引用数 |
129
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME8447
ME8448
ME8451 |
