RRC ID |
29870
|
著者 |
Sullivan MA, Bock RM.
|
タイトル |
Isolation and characterization of antisuppressor mutations in Escherichia coli.
|
ジャーナル |
J Bacteriol
|
Abstract |
Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT.
|
巻・号 |
161(1)
|
ページ |
377-84
|
公開日 |
1985-1-1
|
DOI |
10.1128/jb.161.1.377-384.1985
|
PMID |
3918006
|
PMC |
PMC214882
|
MeSH |
Base Sequence
Codon
Escherichia coli / genetics*
Escherichia coli Proteins
Genes, Bacterial
Lac Operon
Mutation*
Pseudouridine
RNA, Transfer / genetics
RNA, Transfer, Amino Acyl / genetics
Ribosomal Protein S9
Suppression, Genetic*
Transduction, Genetic
beta-Galactosidase / biosynthesis
beta-Galactosidase / genetics
|
IF |
3.006
|
引用数 |
10
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
ME8386
ME8387
ME8388
ME8389
ME8390
ME8391 |