RRC ID |
30025
|
著者 |
Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N.
|
タイトル |
New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
|
ジャーナル |
Gene
|
Abstract |
We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described.
|
巻・号 |
32(3)
|
ページ |
369-79
|
公開日 |
1984-12-1
|
DOI |
10.1016/0378-1119(84)90012-x
|
PII |
0378-1119(84)90012-X
|
PMID |
6099322
|
MeSH |
DNA Transposable Elements*
Escherichia coli / genetics*
Genetic Engineering / methods*
Genetic Vectors
Lac Operon*
Mutation*
Plasmids
beta-Galactosidase / genetics
|
IF |
2.984
|
引用数 |
593
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
原核生物(大腸菌) |
ME9018 |