RRC ID |
30038
|
著者 |
Sadler JR, Tecklenburg M, Betz JL.
|
タイトル |
Plasmids containing many tandem copies of a synthetic lactose operator.
|
ジャーナル |
Gene
|
Abstract |
Up to 12 tandem copies of the lactose operator sequence AATTCCACATGTGGAATTGTGAGCGGATAACAATTTGTGG (3') GGTGTACACCTTAACACTCGCCTATTGTTAAACACCTTAA (5') have been cloned in the EcoRI site of plasmid pMB9. A 12-operator plasmid is about 8% operator by weight and represents a rich source of this DNA segment. A procedure for the rapid and convenient isolation of operator in mg quantities is presented. The lifetimes of complexes formed between repressor and oligo-operator plasmids increased with increasing numbers of tandem operators per plasmid. Evidence is presented indicating that only one tetrameric repressor molecule binds strongly to a segment of four (or fewer) tandem operators, but that two repressor molecules can be accommodated on segments containing at least six tandem operators.
|
巻・号 |
8(3)
|
ページ |
279-300
|
公開日 |
1980-2-1
|
DOI |
10.1016/0378-1119(80)90005-0
|
PII |
0378-1119(80)90005-0
|
PMID |
6244215
|
MeSH |
Base Sequence
DNA Restriction Enzymes / metabolism
DNA, Recombinant / analysis*
Electrophoresis
Escherichia coli / genetics
Lac Operon*
Plasmids*
|
IF |
2.984
|
引用数 |
150
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
原核生物(大腸菌) |
ME8134
ME8135
ME8136
ME8133 |